Protein and Peptide Microarray- Based Assay Technology
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The vast majority of the current technology in the field of microarrays focuses on the field of genomics, with gene expression being the area of greatest commitment, leaving the field of protein microarrays still in its infancy. In fact, a recently published book on microarray analysis dedicates only two pages under the chapter of novel microarray technologies to the topic of protein microarrays (1). The use of antibodybased microarrays for the detection of both research and clinically relevant markers has increased in popularity over the last several years, as protein-compatible surfaces have become commercially available. As with all new technologies, there are multiple steps in the process of producing protein microarrays that still need to be proven. An overview of the process, from the printing of the microarrays through the testing and validation of a system follows: Printing of microarrays can be described as the deposition of a known volume of solution onto a solid substrate. Printers can be of two basic classes; contact or contactless. By far the largest number of printers available on the market are contact printers.
KeywordsAlexa Fluor Protein Microarrays Peptide Array Contact Printer Source Plate
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