The Yeast Two-Hybrid System for Detecting Interacting Proteins

  • Ilya G. Serebriiskii
  • Erica A. Golemis
  • Peter Uetz
Part of the Springer Protocols Handbooks book series (SPH)


Protein-protein interactions play an essential role in all living systems, and hence their analysis is of foremost importance in molecular biology. Although there are num-ber of methods to detect protein-protein interactions, the yeast two-hybrid system is probably the most successful method. Recently established protein interaction data- bases draw their data to a large extent from the summed input of small and large-scale two-hybrid screens.


Library Plasmid Library Screen Bait Protein Bait Plasmid Master Plate 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Ilya G. Serebriiskii
    • 1
  • Erica A. Golemis
    • 2
  • Peter Uetz
    • 2
  1. 1.Division of Basic ScienceFox Chase Cancer CenterPhiladelphia
  2. 2.Institut fur GenetikForschungszentrum KarlsruheGermany

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