Identification of Posttranslational Modification by Mass Spectrometry

  • Alastair Aitken
Part of the Springer Protocols Handbooks book series (SPH)


Many chemically distinct types of posttranslational modification of proteins are known. These include the wide variety of prosthetic groups at enzymes’ active sites, acylations at the N- and the C-terminus and at internal sites in proteins. In this chapter, the examples of mass-spectrometry techniques employed for analysis of glycosylation and disulphide bonds are given. Techniques for identification of sites of phosphorylation are in Chapter 44.


Disulphide Bond Cyanogen Bromide Elution Position Neutral Loss Scanning Protein Prospector 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Alastair Aitken
    • 1
  1. 1.School of Biomedical and Clinical Laboratory SciencesUniversity of EdinburghUK

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