Peptide Sequences of 2-D Gel-Separated Protein Spots by Nanoelectrospray Tandem Mass Spectrometry

  • Alastair Aitken
Part of the Springer Protocols Handbooks book series (SPH)


This chapter describes the analysis of 2-D gel-separated protein spots by nanospray or nanoelectrospray tandem mass spectrometry (MS). The samples may also have originated from one-dimensional gel electrophoresis separations, or from micro- or nanobore multidimensional chromatography. The principles of the technique described below to obtain nanospray into an ion trap or into hybrid MS instruments are similar (1).


Capillary Action Strong Cation Exchange Aqueous Formic Acid Mass Spectrometry Instrument Nanospray Source 
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  1. 1.
    Jensen, O, N., Wilm, M., Shevchenko, A., and Mann, M. (1999) Peptide sequencing of 2-DE gel-isolated proteins by nanoelectrospray tandem mass spectrometry. Methods Mol. Biol. 112, 571–588.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Alastair Aitken
    • 1
  1. 1.School of Biomedical and Clinical Laboratory SciencesUniversity of EdinburghUK

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