Comparing 2-D Electrophoretic Gels Across Internet Databases

An Open Source Application
  • Peter F. Lemkin
  • Gregory C. Thornwall
  • Jai Evans
Part of the Springer Protocols Handbooks book series (SPH)


In (1, 2, 3, 4) and in the previous edition of this book (5), we described a Web-based computer-assisted visual method called Flicker for comparing two-dimensional (2-D) protein gel images across the Internet using a Java applet. We originally used the flicker method in the GELLAB 2-D gel analysis system (6–x9 9). The applet (primarily for Web-based analysis using a Web browser) has since been converted to a Java standalone application that can run on a user’s computer. The new application also can access images from the Web but can now more easily access the user’s data on their local computer. The new version is available as open source on Flicker, where the executable program and the source code are available. Some of the code was derived from the old Flicker applet program and some from the MicroArray Explorer (10)—an open-source data-mining tool for microarray analysis (http://


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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Peter F. Lemkin
    • 1
  • Gregory C. Thornwall
    • 2
  • Jai Evans
    • 3
  1. 1.LECBNCI- FrederickFrederick
  2. 2.LECBSAIC FrederickFrederick
  3. 3.LECBCIT, NIHFrederick

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