2-DE Databases on the World Wide Web

  • Christine Hoogland
  • Khaled Mostaguir
  • Ron D. Appel
Part of the Springer Protocols Handbooks book series (SPH)


With the development of the Internet, a growing number of two-dimensional gel electrophoresis (2-DE) databases become available (50 in January 2004, for a total of 270 image maps). By linking the two components constituting 2-DE databases—images and protein information—the active hypertext links provide a powerful tool for data integration, in addition to navigation from one database to another. This chapter shows how to remotely retrieve data from these databases over the Internet.


Result Page Query Engine Full Text Search Master Image Protein List 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Appel, R. D., Bairoch, A., Sanchez, J. C., et al. (1996) Federated 2-DE database: a simple means of publishing 2-DE data. Electrophoresis 17, 540–546.PubMedCrossRefGoogle Scholar
  2. 2.
    Gasteiger, E., Gattiker, A., Hoogland, C., Ivanyi, I., Appel, R., and Bairoch, A. (2003) ExPASy the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 31, 3784–3788.PubMedCrossRefGoogle Scholar
  3. 3.
    Hoogland, C., Sanchez, J. C., Walther, D., et al. (1999) Two-dimensional electrophoresis resources available from ExPASy. Electrophoresis 20, 3568–3571.PubMedCrossRefGoogle Scholar
  4. 4.
    Hoogland, C., Mostaguir, K., Sanchez, J. C., Hochstrasser, D. F., and Appel, R. D. (2000) SWISS-2DPAGE, ten years later. Proteomics 4, 2352–2356.CrossRefGoogle Scholar
  5. 5.
    Boeckmann, B., Bairoch, A., Apweiler, R., et al. (2003) The SWISS-PROT protein knowledgebase and its supplement TrEMBL in 2003. Nucleic Acids Res. 31, 365–370.PubMedCrossRefGoogle Scholar
  6. 6.
    Gasteiger, E., Jung, E., and Bairoch, A. (2001) SWISS-PROT: connecting biological knowledge via a protein database. Curr. Issues Mol. Biol. 3, 47–55.PubMedGoogle Scholar
  7. 7.
    Bjellqvist, B., Hughes, G. J., Pasquali, C., et al. (1993) The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Elec-trophoresis 14, 1023–1031.CrossRefGoogle Scholar
  8. 8.
    Bjellqvist, B., Basse, B., Olsen, E., and Celis, J. E. (1994) Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions. Electrophoresis 15, 529–539.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Christine Hoogland
    • 1
  • Khaled Mostaguir
    • 1
  • Ron D. Appel
    • 2
  1. 1.Swiss Institute of BioinformaticsGenevaSwitzerland
  2. 2.Swiss Institute of BioinformaticsUniversity and Geneva University HospitalGenevaSwitzerland

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