Silver Staining of 2-D Gels

  • Julia Poland
  • Thierry Rabilloud
  • Pranav Sinha
Part of the Springer Protocols Handbooks book series (SPH)


Proteomics, in its commonly used meaning, refers to the detection of differentially expressed proteins between control and experimental samples. The basic tools include two-dimensional gel electrophoresis (2-DE) for protein fractionation followed by mass spectrometry (MS) of excised protein spots for protein identification. After separation, proteins need to be detected on the gel, meaning the spot pattern has to be distinguished from the surrounding background. This is generally performed directly in the polyacrylamide gel by binding a dye molecule to the proteins.


Silver Staining Silver Nitrate Stop Solution Spot Pattern Silver Sulfide 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Julia Poland
    • 1
  • Thierry Rabilloud
    • 2
  • Pranav Sinha
    • 1
  1. 1.Institut fur Medizinische und Chemische LabordiagnostikLandeskrankenhaus KlagenfurtKlagenfurtAustria
  2. 2.DBMS/BECPCEA-GrenobleFrance

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