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Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis

  • William L. Godfrey
  • Colette J. Rudd
  • Sujata Iyer
  • Diether Recktenwald
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

A goal of proteomic research is to detect all proteins in a particular biological subsystem, such as a cell type or cellular subfraction (1). This effort requires a combination of technologies first, for subfractionation and purification of the cells and cellular components of interest, and second, a sensitive method for detection and identification of all possible proteins. The challenge of proteomics is the wide range of abundance and sizes of cellular proteins, as well as the number of different proteins and posttranslational modifications. Clearly, maximizing both the specificity of the sample purification method and the sensitivity of the protein detection method is essential. In addition, targeting the analysis to specific sub-components of the cell can both enhance the sensitivity of the analysis and contribute to the functional analysis of the proteins (2).

Keywords

Side Scatter Unlabeled Cell Instrument Setup Digestion Medium Normal Human Blood 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • William L. Godfrey
    • 1
  • Colette J. Rudd
    • 2
  • Sujata Iyer
    • 2
  • Diether Recktenwald
    • 3
  1. 1.Molecular Probes, Inc.Eugene
  2. 2.BD BiosciencesSan Jose
  3. 3.Research Department, BD BiosciencesSan Jose

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