Peptide Synthesis and Applications pp 227-240

Part of the Methods in Molecular Biology™ book series (MIMB, volume 298)

Protein Identification by Mass Spectrometric Analyses of Peptides

  • Ashley Martin
Protocol

Abstract

The focus of this chapter is the isolation and analysis of subsets of proteins or subproteomes. These methodologies can be applied to address fundamental questions in many areas of biological research. Protocols are provided for common techniques that include protein isolation from mammalian cells and the subsequent identification of peptides derived from endogenous proteins by the action of proteases.

Key Words

Mass spectrometry proteome SDS-PAGE trypsin digest cell lysis 

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Ashley Martin
    • 1
  1. 1.Cancer Research UK Institute for Cancer StudiesUniversity of BirminghamBirmingham

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