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Introduction to Capillary Electrophoresis of DNA

Biomedical Applications
  • Beatriz Sanchez-Vega
Protocol
  • 1.4k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Capillary electrophoresis (CE) separations are carried out inside a capillary tube, which usually has a diameter of 50 μm to facilitate temperature control. The length of the capillary differs in different applications, but it is typically in the region of 20–50 cm. The capillaries most widely used are fused silica covered with an external protective coating. A small portion of this coating is removed to form a window for detection purposes. The ends of the capillary are dipped into reservoirs filled with the electrolyte. Electrodes made of an inert material such as platinum are also inserted into the electrolyte reservoirs to complete the electrical circuit. The capillary is filled with running buffer, one end is dipped into the sample, and an electric field (electrokinetic injection) or pressure is applied to introduce the sample inside the capillary. Migration through the capillary is driven by application of a high-voltage current (10–30 kV).

Keywords

Capillary Electrophoresis Congenital Adrenal Hyperplasia Peptide Nucleic Acid Hereditary Hemochromatosis Heteroduplex Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Beatriz Sanchez-Vega
    • 1
  1. 1.University of Texas MD Anderson Cancer CenterHouston

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