Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing

  • Anu Suomalainen
  • Ann-Christine Syvänen
Part of the Springer Protocols Handbooks book series (SPH)


The PCR technique provides a highly specific and sensitive means for analyzing nucleic acids, but it does not allow their direct quantification. This limitation is because the efficiency of PCR depends on the amount of template sequence present in the sample, and the amplification is exponential only at low template concentrations (1). Owing to this plateau effect of PCR, the amount of amplification product does not directly reflect the original amount of template. Moreover, subtle differences in reaction conditions, such as material from biological samples, may cause significant sample-to-sample variation in the final yield of the PCR product.


Molecular Genetic Laboratory Molecular Beacon Probe Determine Allele Frequency Minisequencing Reaction Scintillate Polystyrene 
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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Anu Suomalainen
    • 1
  • Ann-Christine Syvänen
    • 2
  1. 1.Programme of Neurosciences and Department of NeurologyHelsinki UniversityHelsinkiFinland
  2. 2.Department of Medical SciencesUppsala UniversityUppsalaSweden

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