Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis

  • Tuan Vo-Dinh
  • Fei Yan
  • David L. Stokes
Part of the Methods in Molecular Biology™ book series (MIMB, volume 300)


Surface-enhanced Raman scattering (SERS) spectroscopy is a plasmonics-based spectroscopic technique that combines modern laser spectroscopy with unique optical properties of metallic nanostructures, resulting in strongly increased Raman signals when molecules are adsorbed on or near nanometer-size structures of special metals such as gold, silver, and transition metals. This chapter provides a synopsis of the development and application of SERS-active metallic nanostructures, especially for the analysis of biologically relevant compounds. Some highlights of this chapter include reports of SERS as an immunoassay readout method, SERS gene nanoprobes, near-field scanning optical microscopy SERS probes, SERS as a tool for single-molecule detection, and SERS nanoprobes for cellular studies.

Key Words

Surface-enhanced Raman scattering genomics single-molecule detection near-field scanning optical microscopy plasmonics bioanalysis 



This work was jointly sponsored by the Federal Bureau of Investigation (Project No. 2051-II18-Y1), and the Office of Biological and Environmental Research, U.S. Department of Energy, under contract DE-AC05-00OR22725 with UT-Battelle, LLC; and by the Laboratory Directed Research and Development Program (Advanced Plasmonics Sensors Project) at Oak Ridge National Laboratory. Fei Yan and David L. Stokes are also supported by an appointment to the Oak Ridge National Laboratory Postdoctoral Research Associates Program, administered jointly by the Oak Ridge National Laboratory and Oak Ridge Institute for Science and Education.


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Copyright information

© Humana Press Inc., Totowa, NJ 2005

Authors and Affiliations

  • Tuan Vo-Dinh
    • 1
  • Fei Yan
    • 2
  • David L. Stokes
    • 2
  1. 1.Center for Advanced Biomedical Photonics, Life Sciences DivisionOak Ridge National LaboratoryOak Ridge
  2. 2.Center for Advanced Biomedical PhotonicsOak Ridge National LaboratoryOak Ridge

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