DNA Viruses pp 267-276

Part of the Methods in Molecular Biology book series (MIMB, volume 292)

DNA Affinity Purification of Epstein-Barr Virus OriP-Binding Proteins

  • Constandache Atanasiu
  • Larissa Lezina
  • Paul M. Lieberman
Protocol

Abstract

DNA affinity purification has been used to identify cellular and viral proteins associated with the Epstein-Barr virus origin of plasmid DNA replication. This approach allows for a one- or two-step purification scheme of high-affinity DNA binding proteins from crude nuclear extracts. Additionally, this approach may be useful for isolation of proteins that are found in the insoluble fractions of the nuclear matrix or scaffold.

Key Words

DNA affinity magnetic beads OriP EBNA-1 telomere repeat binding factors 

Copyright information

© Humana Press Inc. 2005

Authors and Affiliations

  • Constandache Atanasiu
    • 1
  • Larissa Lezina
    • 2
  • Paul M. Lieberman
    • 2
  1. 1.The Wistar InstitutePhiladelphia
  2. 2.The Wistar InstitutePhiladelphia

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