Cell Cycle Control and Dysregulation Protocols pp 37-40

Part of the Methods in Molecular Biology™ book series (MIMB, volume 285) | Cite as

Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins

  • Amjad Javed
  • Sayyed K. Zaidi
  • Soraya E. Gutierrez
  • Christopher J. Lengner
  • Kimberly S. Harrington
  • Hayk Hovhannisyan
  • Brian C. Cho
  • Jitesh Pratap
  • Shirwin M. Pockwinse
  • Martin Montecino
  • André J. van Wijnen
  • Jane B. Lian
  • Janet L. Stein
  • Gary S. Stein

Abstract

Only a handful of tagged molecules have been used successfully in vivo to monitor gene expression. The most commonly used are β-galactosidase (β-gal) and green fluorescent protein (GFP). Both molecules pose limitations with in vivo detection. When using GFP, autofluorescence may be encountered in tissues and tissue sections. Similarly, when using β-gal as a marker, the penetration of X-gal substrate is difficult in tissues with extensive extra cellular matrix and in calcified tissues.

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Amjad Javed
    • 1
  • Sayyed K. Zaidi
    • 1
  • Soraya E. Gutierrez
    • 1
  • Christopher J. Lengner
    • 1
  • Kimberly S. Harrington
    • 1
  • Hayk Hovhannisyan
    • 1
  • Brian C. Cho
    • 1
  • Jitesh Pratap
    • 1
  • Shirwin M. Pockwinse
    • 1
  • Martin Montecino
    • 1
  • André J. van Wijnen
    • 1
  • Jane B. Lian
    • 1
  • Janet L. Stein
    • 1
  • Gary S. Stein
    • 1
  1. 1.Department of Cell BiologyUniversity of Massachusetts Medical SchoolWorcester

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