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Measurement of Apoptotic Cell Clearance In Vitro

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Part of the Methods in Molecular Biology book series (MIMB,volume 282)

Abstract

The phagocytic clearance of apoptotic cells is a highly efficient and non-phlogistic process in vivo. Research in this area has been limited, at least in part, by technical difficulties associated with the techniques used in the detailed study of apoptotic cell clearance mechanisms. This chapter provides details of methods that may be used to study apoptotic cell clearance in vitro. Such methods have been used successfully to identify phagocyte-associated or apoptotic cell-associated molecular players in the recognition process.

Key Words

  • Macrophage
  • phagocyte
  • apoptotic cell clearance
  • phagocytosis

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  • DOI: 10.1385/1-59259-812-9:207
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References

  1. Devitt, A., Moffatt, O. D., Raykundalia, C., Capra, J. D., Simmons, D. L., and Gregory, C. D. (1998). Human CD14 mediates recognition and phagocytosis of apoptotic cells. Nature 392, 505–509.

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  2. Moffatt, O. D., Devitt, A., Bell, E. D., Simmons, D. L., and Gregory, C. D. (1999). Macrophage recognition of ICAM-3 on apoptotic leukocytes. J. Immunol. 162, 6800–6810.

    PubMed  CAS  Google Scholar 

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© 2004 Humana Press Inc.

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Devitt, A., Gregory, C.D. (2004). Measurement of Apoptotic Cell Clearance In Vitro. In: Brady, H.J.M. (eds) Apoptosis Methods and Protocols. Methods in Molecular Biology, vol 282. Humana Press. https://doi.org/10.1385/1-59259-812-9:207

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  • DOI: https://doi.org/10.1385/1-59259-812-9:207

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-873-8

  • Online ISBN: 978-1-59259-812-0

  • eBook Packages: Springer Protocols