Apoptosis Methods and Protocols pp 179-189

Part of the Methods in Molecular Biology book series (MIMB, volume 282)

Measuring Programmed Cell Death in Plants

  • Ludmila Rizhsky
  • Vladimir Shulaev
  • Ron Mittler

Abstract

Methods for the detection of programmed cell death (PCD) in plants are reviewed with references for different biochemical, microscopic, and molecular assays. A detailed description of three different methods for the detection of biotic or abiotic PCD in plant tissues is included. The reader is encouraged to use all three methods in parallel to obtain a reliable measure of PCD. Critical considerations are highlighted.

Key Words

Programmed cell death plant hypersensitive response nuclease DNA degradation ion lekeage 

Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Ludmila Rizhsky
    • 1
  • Vladimir Shulaev
    • 2
  • Ron Mittler
    • 3
  1. 1.Department of Botany, Plant Sciences InstituteIowa State UniversityAmes
  2. 2.Virginia Bioinformatics InstituteBlacksburg
  3. 3.Department of BiochemistryUniversity of NevadaReno

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