Lentiviral-Mediated Gene Transfer to Model Triplet Repeat Disorders

  • Etienne Régulier
  • Diana Zala
  • Patrick Aebischer
  • Nicole Déglon
Part of the Methods in Molecular Biology™ book series (MIMB, volume 277)


This chapter describes the potential use of viral-mediated gene transfer in the central nervous system as a new strategy in developing animal models of neurodegenerative diseases. To illustrate the approach, procedures for the production of lentiviral vectors encoding polyQ proteins are provided, as well as methods for the determination of viral titers, in vitro infection, and basic protocols for in vivo studies in rodents.

Key Words

Gene transfer brain lentiviral vectors primary cultures animal models rodent 


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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Etienne Régulier
    • 1
  • Diana Zala
    • 1
  • Patrick Aebischer
    • 1
  • Nicole Déglon
    • 1
  1. 1.Institute of Neuroscience, School of Life SciencesSwiss Federal Institute of Technology Lausanne (EPFL)LausanneSwitzerland

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