High-Throughput Expression in Microplate Format in Pichia pastoris

  • Mewes Böttner
  • Christine Lang
Part of the Methods in Molecular Biology book series (MIMB, volume 267)

Abstract

The methylotrophic yeast Pichia pastoris has become a powerful host for the heterologous expression of proteins. To serve the increasing demand for clones expressing different cDNAs, we developed a cultivation and induction protocol amenable to automation to increase the number of clones screened for protein expression. Therefore cDNAs are cloned for intracellular expression. The resulting fusion proteins carry affinity tags (6*HIS and StrepII, respectively) at the N- and C-terminus for the immunological detection and chromatographic purification of full-length proteins. Expression is controlled by the tightly regulated and highly inducible alcohol oxidase 1 (AOX1) promoter. The screening procedure is based on a culture volume of 2 mL in a 24-well format. Lysis of the cells occurs via chemical lysis without mechanical disruption. Using the optimized feeding and induction protocol, we are now able to screen for and identify expression clones that produce heterologous protein with a yield of 2 mg per L culture volume or higher.

Key Words

Pichia pastoris posttranslational modifications expression screening microplates AOX1 promoter 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Mewes Böttner
    • 1
  • Christine Lang
    • 1
  1. 1.Berlin Technical UniversityInstitute for BiotechnologyBerlinGermany

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