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Construction of Small Genome BAC Library for Functional and Genomic Applications

  • David Šmajs
  • Steven J. Norris
  • George M. Weinstock
Part of the Methods in Molecular Biology™ book series (MIMB, volume 255)

Abstract

The use of genetic approaches to study bacteria is limited by the following considerations: first, the bacteria must be easy to culture and nonpathogenic; and, second, a broad spectrum of genetic techniques for the particular bacteria being studied must be available. For other bacteria, such as the syphilis-causing spirochete, Treponema pallidum, genetic studies are highly limited because the bacterium cannot be continuously grown in the laboratory, because of its high virulence and a total lack of genetic tools to study this organism.

Keywords

Bacterial Artificial Chromosome Luria Bertani Bacterial Artificial Chromosome Clone Bacterial Artificial Chromosome Library White Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • David Šmajs
    • 1
  • Steven J. Norris
    • 2
  • George M. Weinstock
    • 3
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of Texas-Houston Medical SchoolHouston
  2. 2.Department of Pathology and Laboratory MedicineUniversity of Texas-Houston Medical SchoolHouston
  3. 3.Human Genome Sequencing CenterBaylor College of MedicineHouston

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