mRNA Processing and Metabolism pp 29-46
Imaging Alternative Splicing in Living Cells
We have developed an in vivo reporter of alternative splicing decisions that allows for the determination of FGF-R2 splicing patterns without the destruction of cells. This method has broad applications, including the study of other alternatively spliced genes in tissue culture and in whole animals, and may be useful in creating imaging markers for the study of tumor progression and metastasis. In this chapter, the authors present one example of this method using fluorescence reporters. As with any new assay, a series of experiments were performed to validate the method. This chapter documents some of these experiments.
Key WordsAlternative splicing in vivo imaging fluorescence intronic splicing silencers green fluorescent protein fibroblast growth factor receptor