Optical Coherence Tomography Imaging in Developmental Biology
Part of the Methods in Molecular Biology™ book series (MIMB, volume 135)
Optical coherence tomography (OCT) is an attractive imaging technique for developmental biology because it permits the imaging of tissue microstructure in situ, yielding micron-scale image resolution without the need for excision of a specimen and tissue processing. OCT enables repeated imaging studies to be performed on the same specimen in order to track developmental changes. OCT is analogous to ultrasound B mode imaging except that it uses low-coherence light rather than sound and performs cross-sectional imaging by measuring the backscattered intensity of light from structures in tissue (1). The principles of OCT imaging are shown schematically in Fig. 1. The OCT image is a gray-scale or false-color two-dimensional (2-D) representation of backscattered light intensity in a cross-sectional plane. The OCT image represents the differential backscattering contrast between different tissue types on a micron scale. Because OCT performs imaging using light, it has a one- to two-order-of-magnitude higher spatial resolution than ultrasound and does not require specimen contact.
KeywordsOptical Coherence Tomography Optical Coherence Tomography Image Axial Resolution Optical Coherence Tomography System Forsterite Laser
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- 4.Puliafito, C. A., Hee, M. R., Schuman, J. S., and Fujimoto, J. G. (1995) Optical Coherence Tomography of Ocular Diseases. Slack, Thorofare, NJ.Google Scholar
- 9.Sergeev, A., Gelikonov, B., Gelikonov, G., Feldchetin, F., Pravdenki, K., Kuranov, R., et al. (1995) High-spatial resolution optical-coherence tomography of human skin and mucus membranes in Conference on Lasers and Electro Optics ‘95, Vol. 15 of 1995 OSA Technical Digest Series (Optical Society of America, Washington, DC), paper CThN4.Google Scholar
- 12.Brezinski, M. E., Tearney, G. J., Weissman, N. J., Boppart, S. A., Bouma, B. E., Hee, M. R., et al. (1997) Assessing atherosclerotic plaque morphology: Comparison of optical coherence tomography and high frequency intravascular ultrasound. Br. Heart J. 77, 397–404.Google Scholar
- 20.de Boer, J. F., Milner, T. E., van Germert, M. J. C., and Stuart Nelson, J. (1997) Two dimensional birefringence imaging in biological tissue by polarization sensitive optical coherence tomography. Opt. Lett. 22, 934–936.Google Scholar
- 29.Chernikov, S. V., Zhu, Y., Taylor, J. R., Platonov, N. S., Samartsev, I. E., and Gapontsev, V. P. (1996) 1.08–2.2 μm supercontinuum generation from Yb3+ doped fiber laser. Conference on Lasers and Electro Optics CLEO 96, Vol. 9 of 1996 OSA Technical Digest Series (Optical Society of America, Washington, DC) paper CTuU4.Google Scholar
- 30.Swanson, E. A., Chinn, S. R., Hodgson, C. W., Bouma, B. E., Tearney, G. J., and Fujimoto, J. G. (1996) Spectrally shaped rare-earth doped fiber ASE sources for use in optical coherence tomography. Conference on Lasers and Electro Optics CLEO 96, Vol. 9 of OSA 1996 Technical Digest, (Optical Society of America, Washington, DC) paper CTuU5.Google Scholar
- 31.Chernikov, V., Taylor, J. R., Gapontsev, V. P., Bouma, B. E., and Fujimoto, J. G. (1997) A 75 nm, 30 mW superfluorescent ytterbium fiber source operation around 1.06 μm. Conference on Lasers and Electro Optics CLEO 97, Vol. 11 of OSA 1997 Technical Digest, (Optical Society of America, Washington, DC) paper CTuG8.Google Scholar
- 35.Nieuwkoop, P. D. and Faber, J. (1994) Normal Table of Xenopus Laevis. Garland, New York.Google Scholar
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