Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes

  • Maurice S. Swanson
  • Gideon Dreyfuss
Part of the Methods in Molecular Biology™ book series (MIMB, volume 118)

Abstract

Proper formation of different types of ribonucleoprotein (RNP)-RNA complexes is critical for the correct expression of genetic information at the posttranscriptional level. In the nucleus, RNA polymerase II transcripts bind to an abundant class of nuclear pre-mRNA/mRNA-binding proteins, the heterogeneous nuclear ribonucleoproteins (hnRNPs), to form hnRNP complexes (1,2). The molecular architecture of these complexes must be exceedingly dynamic because each step in the pre-mRNA processing pathway alters the structure of the underlying RNA substrate. Therefore, hnRNP complexes within the cell, although probably unique for each transcript, are not uniform structures, such as a ribosomal subunit or a particular small nuclear ribonucleoprotein particle (snRNP), but are composed of a heterogeneous mixture of protein-RNA structures.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Maurice S. Swanson
    • 1
  • Gideon Dreyfuss
    • 2
  1. 1.Department of Molecular Genetics and Microbiology and Centers for Gene Therapy and Mammalian GeneticsUniversity of Florida College of MedicineGainesvilleUSA
  2. 2.Howard Hughes Medical Institute, Department of Biochemistry and BiophysicsUniversity of Pennsylvania School of MedicinePhiladelphiaUSA

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