Cytokine Protocols pp 201-218

Part of the Methods in Molecular Biology book series (MIMB, volume 249)

Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level

  • Karin Loré
  • Jan Andersson

Abstract

Methods for detection of cytokines and chemokines at a protein level in individual cells based on immunofluorescence or immunohistochemical (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16) staining techniques have been developed in our laboratory. The difficulties in these procedures lie in the critical steps of fixation and permeabilization of the cells in combination with a very careful selection of suitable cytokine- or chemokine-specific antibodies.

Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Karin Loré
    • 1
  • Jan Andersson
    • 1
  1. 1.Center for Infectious Medicine, Department of Medicine, Karolinska InstitutetHuddinge University HospitalStockholmSweden

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