Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy
Fluorescence spectroscopy is a widely used technique for the characterization of molecular interactions in biological systems. It is highly sensitive, and allows measurements at low sample concentrations (down to 10-7 M). It is also available at a reasonably low equipment cost. These features make fluorescence spectroscopy an attractive technique as compared to other forms of optical spectroscopy.
- 1.Eftink, M. R. (1997) in Methods in Enzymology: Fluorescence Spectroscopy (Brand, L. and Johnson, M. L., eds.), Academic Press, New York, NY, pp. 278, pp. 221–257.Google Scholar
- 5.Parker, C. W. (1978) Spectrofluorometric methods, in Experimental Immunology, 3rd ed., Blackwell Scientific Publications, Oxford UK, pp. 1–25.Google Scholar