Antibody Engineering pp 431-441
Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy
Fluorescence spectroscopy is a widely used technique for the characterization of molecular interactions in biological systems. It is highly sensitive, and allows measurements at low sample concentrations (down to 10-7M). It is also available at a reasonably low equipment cost. These features make fluorescence spectroscopy an attractive technique as compared to other forms of optical spectroscopy.