Antibody Engineering pp 431-441

Part of the Methods in Molecular Biology™ book series (MIMB, volume 248)

Characterization of Antibody-Antigen Interactions by Fluorescence Spectroscopy

  • Sotiris Missailidis
  • Kevin Brady


Fluorescence spectroscopy is a widely used technique for the characterization of molecular interactions in biological systems. It is highly sensitive, and allows measurements at low sample concentrations (down to 10-7M). It is also available at a reasonably low equipment cost. These features make fluorescence spectroscopy an attractive technique as compared to other forms of optical spectroscopy.

Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Sotiris Missailidis
    • 1
  • Kevin Brady
    • 2
  1. 1.Department of ChemistryThe Open UniversityMilton KeynesUK
  2. 2.School of ChemistryUniversity of NottinghamNottinghamUK

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