G Protein Signaling pp 21-38

Part of the Methods in Molecular Biology™ book series (MIMB, volume 237)

Purification of G Protein Subunits from Sf9 Insect Cells Using Hexahistidine-Tagged α and βγ Subunits

  • Tohru Kozasa

Summary

G protein-mediated pathways are the most fundamental mechanisms of cell signaling. In order to analyze these pathways, the availability of purified recombinant G proteins are critically important. Using Sf9-Baculovirus expression system, a general and simplified method to purify various G protein subunits is described in this chapter. This method is useful for purification of most of G protein subunits.

Key Words

G protein recombinant protein Sf9-Baculovirus expression system 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Tohru Kozasa
    • 1
  1. 1.Department of PharmacologyUniversity of Illinois at ChicagoChicago

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