Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry

  • Sukriti Nag
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 89)


Transmission electron microscopy (TEM), a once popular research tool, is used less frequently now. However, the isolation of novel proteins in the past decade has led to renewed use of electron microscopy for the subcellular localization of these proteins. This chapter will describe the standard method for preparation of brain tissue for TEM studies of cerebral vessels.


Ultrathin Section Osmium Tetroxide Cacodylate Buffer Propylene Oxide Fume Hood 
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  1. 1.
    Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A.Google Scholar
  2. 2.
    Hayat, M. A. (2000) Principles and Techniques of Electron Microscopy. Biological Applications, 4th ed. Cambridge University Press, New York.Google Scholar
  3. 3.
    Hunter E. (1993) Practical Electron Microscopy. A Beginner’s Illustrated Guide, 2nd ed. Cambridge University Press, Cambridge, UK.Google Scholar
  4. 4.
    Peachey, L. D. (1958) Thin sections. I. A study of section thickness and physical distortion produced during microtomy. J. Biophys. Biochem. Cytol. 4, 233–242.PubMedCrossRefGoogle Scholar
  5. 5.
    Baumbach, G. L., Walmsley, J. G., and Hart, M. N. (1988) Composition and mechanics of cerebral arterioles in hypertensive rats. Am. J. Pathol. 133, 464–471.PubMedGoogle Scholar
  6. 6.
    Gross, P. M., Sposito, N. M., Pettersen, S. E., and Fenstermacher, J. D. (1986) Differences in function and structure of the capillary endothelium in gray matter, white matter and a circumventricular organ of rat brain. Blood Vessels 23, 261–270.PubMedGoogle Scholar
  7. 7.
    Nag, S., and Kilty, D. W. (1997) Cerebrovascular changes in chronic hypertension. Protective effects of enalapril in rats. Stroke 28, 1028–1034.PubMedGoogle Scholar
  8. 8.
    Nag, S. (1995) Role of endothelial cytoskeleton in blood-brain barrier permeability to protein. Acta Neuropathol. (Berl) 90, 454–460.CrossRefGoogle Scholar
  9. 9.
    Nag S. (1998) Blood-brain barrier permeability measured with histochemistry. In Introduction to the Blood-Brain Barrier. Methodology, Biology and Pathology. Pardridge, W. M. ed. Cambridge University Press, Cambridge, UK, pp 113–121.CrossRefGoogle Scholar
  10. 10.
    Stewart, P. A., Hayakawa, K., and Farrell C. (1994) Quantitation of blood-brain barrier ultra-structure. Microsc. Res. Tech. 27, 516–527.PubMedCrossRefGoogle Scholar
  11. 11.
    Nagy, Z., Peters, H., and Huttner, I. (1983) Charge-related alterations of the cerebral endothelium. Lab. Invest. 49, 662–671.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Sukriti Nag
    • 1
    • 2
  1. 1.Division of NeuropathologyUniversity of TorontoTorontoCanada
  2. 2.Toronto Western Research InstituteUniversity Health NetworkTorontoCanada

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