Studies of Cerebral Vessels by Transmission Electron Microscopy and Morphometry
Transmission electron microscopy (TEM), a once popular research tool, is used less frequently now. However, the isolation of novel proteins in the past decade has led to renewed use of electron microscopy for the subcellular localization of these proteins. This chapter will describe the standard method for preparation of brain tissue for TEM studies of cerebral vessels.
KeywordsUltrathin Section Osmium Tetroxide Cacodylate Buffer Propylene Oxide Fume Hood
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