The Blood-Brain Barrier pp 133-144 | Cite as
Blood-Brain Barrier Permeability Using Tracers and Immunohistochemistry
Abstract
The Exogenous Tracers Used to Detect Blood-Brain Barrier Permeability and the Methods to Detect Endogenous Protein Extravasations
I. Exogenous Tracers |
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1. Markers of Protein Permeability |
Dyes: Evans blue |
Fluorescent Tracers: |
Fluorescein isothiocynate-dextran (MW: 62,000) |
Fluorescein isothiocynate-albumin (MW: 67,000) |
Horseradish peroxidase* (MW: 40,000) |
Microperoxidase* (MW: 1900) |
Radiolabeled Compounds |
125Idine-labeled serum albumin |
14C-labeled dextran (MW: 70,000) |
2. Markers of Solute and Ion Permeability |
Ionic Lanthanum* (MW: 138.9) |
Sodium Fluorescein (MW: 376) |
Lucifer Yellow (MW: 457) |
Ruthenium Red* (MW: 800) |
Fluorescein isothiocynate-dextran (MW: 3000) |
Radio-labeled Compounds |
14C-α -Aminoisobutyric acid (MW 103) |
14C Sucrose (MW: 342) |
14C- Methotrexate (MW: 455) |
14C-Inulin (MW: 5000) |
II.Endogenous Protein Extravasation |
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Detected of Plasma Proteins by Immunohistochemistry |
Immunoperoxidase |
Immunofluorescence |
Immunogold technique |
Keywords
Evans Blue Lanthanum Chloride Ionic Lanthanum Acute Hypertension Polyethylene CannulaReferences
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