Metabolite Profiling as a Functional Genomics Tool

  • Anusha P. Dias
  • Johnny Brown
  • Pierluigi Bonello
  • Erich Grotewold
Part of the Methods in Molecular Biology™ book series (MIMB, volume 236)


Plants accumulate a very large number of small molecules (phytochemicals) with important functions in the ecology of plants and in the protection against biotic and abiotic stress conditions. Little is known on how phytochemical biosynthetic pathways are regulated, which is a key step to successfully engineering plant metabolism. Plant natural products are usually not essential, and genetic analyses often fail to identify phenotypes associated with the absence of these compounds. We have investigated the use of metabolite profiling of plant cells in culture to establish the function of transcription factors suspected to control plant metabolic pathways.

Key Words

natural products transcription factors R2R3 Myb culture cells GC/MS HPLC 


  1. 1.
    Braun, E. L., Dias, A. P., Matulnik, T. J., and Grotewold, E. (2001) Transcription factors and metabolic engineering: novel applications for ancient tools. Recent. Adv. Phytochem. 35, 79–109.CrossRefGoogle Scholar
  2. 2.
    Memelink, J., Kijne, J. W., van der Heijden, R., and Verpoorte, R. (2001) Genetic modification of plant secondary metabolite pathways using transcriptional regulators. Adv. Biochem. Eng. Biotechnol. 72, 103–125.PubMedGoogle Scholar
  3. 3.
    Grotewold, E., Chamberlain, M., St. Claire, G., et al. (1998) Engineering secondary metabolism in maize cells by ectopic expression of transcription factors. Plant Cell 10, 721–740.PubMedCrossRefGoogle Scholar
  4. 4.
    Dias, A. P. and Grotewold, E. (2003) Manipulating the accumulation of phenolics in maize cultured cells using transcription factors. Biochem. Eng. J., 14, 207–216.CrossRefGoogle Scholar
  5. 5.
    Fiehn, O., Kopka, J., Dormann, P., Altmann, T., Trethewey, R. N., and Willmitzer, L. (2000) Metabolite profiling for plant functional genomics. Nat. Biotechnol. 18, 1157–1161.PubMedCrossRefGoogle Scholar
  6. 6.
    Roessner, U., Luedemann, A., Brust, D., et al. (2001) Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell 13, 11–29.PubMedCrossRefGoogle Scholar
  7. 7.
    Cannell, R. J. P. (ed.) (1998) Natural Products Isolation. Methods in Biotechnology. Humana Press, Totowa.Google Scholar
  8. 8.
    Glassbrook, N. and Ryals, J. (2001) A systematic approach to biochemical profiling. Curr. Opin. Plant Biol. 4, 186–190.PubMedCrossRefGoogle Scholar
  9. 9.
    Grotewold, E., Drummond, B., Bowen, B., and Peterson, T. (1994) The Myb-homologous P gene controls phlobaphene pigmentation in maize floral organs by directly activating a flavonoid biosynthetic gene subset. Cell 76, 543–553.PubMedCrossRefGoogle Scholar
  10. 10.
    Rosemann, D., Heller, W., and Sandermann, H., Jr. (1991) Biochemical plant responses to ozone II. Induction of stilbene biosysthesis in scots pine (Pinus sylvestris L.) seedlings. Plant Physiol. 97, 1280–1286.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Anusha P. Dias
    • 1
  • Johnny Brown
    • 2
  • Pierluigi Bonello
    • 1
  • Erich Grotewold
    • 1
  1. 1.Department of Plant Biology and Plant Biotechnology CenterThe Ohio State UniversityColumbus
  2. 2.CCIC-MS FacilityThe Ohio State UniversityColumbus

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