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Metabolite Profiling as a Functional Genomics Tool

  • Anusha P. Dias
  • Johnny Brown
  • Pierluigi Bonello
  • Erich Grotewold
Part of the Methods in Molecular Biology™ book series (MIMB, volume 236)

Abstract

Plants accumulate a very large number of small molecules (phytochemicals) with important functions in the ecology of plants and in the protection against biotic and abiotic stress conditions. Little is known on how phytochemical biosynthetic pathways are regulated, which is a key step to successfully engineering plant metabolism. Plant natural products are usually not essential, and genetic analyses often fail to identify phenotypes associated with the absence of these compounds. We have investigated the use of metabolite profiling of plant cells in culture to establish the function of transcription factors suspected to control plant metabolic pathways.

Key Words

natural products transcription factors R2R3 Myb culture cells GC/MS HPLC 

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Copyright information

© Humana Press Inc., Totowa, NJ 2003

Authors and Affiliations

  • Anusha P. Dias
    • 1
  • Johnny Brown
    • 2
  • Pierluigi Bonello
    • 1
  • Erich Grotewold
    • 1
  1. 1.Department of Plant Biology and Plant Biotechnology CenterThe Ohio State UniversityColumbus
  2. 2.CCIC-MS FacilityThe Ohio State UniversityColumbus

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