Extraction of Ancient DNA

  • Wera M. Schmerer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)


The DNA extraction process represents one of the critical stages in the analysis of degraded or ancient DNA. If polymerase chain reaction (PCR) amplification starts from a poor extract containing low template quantities, stochastic variation in the amplification of individual alleles may lead to allelic dropout (1), resulting in a high risk of false-homozygous typing of a heterozygous sample (2).


Allelic Dropout Sodium Perchlorate Bone Meal Bone Powder Safety Data Sheet 
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Wera M. Schmerer
    • 1
  1. 1.Paleo DNA Laboratory, Department of AnthropologyLakehead UniversityThunder BayCanada

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