28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use

  • Phil Lefebvre
  • Isaac Cohen
Part of the Methods in Molecular Biology™ book series (MIMB, volume 215)


This chapter will describe a method used to produce human megakaryocytes in vitro (see Fig. 1), in high yield, for a clinical product to supplement progenitor cell transplants. Producing significant numbers of megakaryocytes from human progenitor cells requires specific combinations of cytokines to promote their proliferation and maturation in vitro. Thrombopoietin (TPO) is the primary regulator of megakaryocytopoiesis (1-6). Other cytokines, including interleukin-3 (IL-3), IL-6, IL-11, stem cell factor (SCF; also known as kit- ligand), and granulocyte-macrophage colony stimulating factor (GM-CSF) are also capable of promoting megakaryocytopoiesis and will synergize with TPO to increase megakaryocyte proliferation (reviewed in ref. 7).


Allogeneic Blood Transfusion Human Megakaryocyte Mature Megakaryocyte Human Progenitor Cell Megakaryocyte Proliferation 
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Phil Lefebvre
    • 1
  • Isaac Cohen
    • 1
  1. 1.Department of MedicineNorthwestern UniversityChicago

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