Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes

  • Anna Shevchenko
  • Shamil Sunyaev
  • Adam Liska
  • Peer Bork
  • Andrej Shevchenko
Part of the Methods in Molecular Biology™ book series (MIMB, volume 211)

Abstract

Recent developments in technology and instrumentation have made mass spectrometry the method of choice for identification of proteins in a sequence database [reviewed in refs. (1, 2, 3, 4)].Regardless of what mass spectrometric technology is applied, it is ultimately required that acquired mass spectra be accurately matched to a protein sequence from the corresponding database entry.

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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Anna Shevchenko
    • 1
  • Shamil Sunyaev
    • 2
  • Adam Liska
    • 1
  • Peer Bork
    • 2
  • Andrej Shevchenko
    • 2
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany
  2. 2.MPI of Molecular Cell Biology and GeneticsDresdenGermany

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