Determination of Cancer Allelotype

  • Jennifer J. Ascaño
  • Steven M. Powell
Part of the Methods in Molecular Biology™ book series (MIMB, volume 222)


The term “allelotype” was first used by Vogelstein and colleagues (1), in analogy to karyotype, to describe a newly developed molecular analysis that surveyed chromosomal loss and/or aberration in a panel of human colorectal tumors. This study was the first to comprehensively screen 39 nonacrocentric chromosomal arms in surveying restriction fragment polymorphisms (RFLPs) using variable number tandem repeats (VNTRs) as probes. Simple tandem repeats, including VNTRs and microsatellite loci consisting of 1- to 5-bp repeats, have been found to be highly polymorphic and relatively stable among the human population, which facilitates the analysis of both maternal and paternal alleles. These markers have also been useful in linkage analysis when studying hereditary human disease (2, 3, 4, 5).


Polymerase Chain Reaction Product Bottom Plate Variable Number Tandem Repeat Acinar Cell Carcinoma Dark Bottle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Jennifer J. Ascaño
    • 1
  • Steven M. Powell
    • 1
  1. 1.Division of Gastroenterology and HepatologyUniversity of Virginia Health SystemCharlottesville

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