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Transformation of Haemophilus influenzae

  • Grant Poje
  • Rosemary J. Redfield
Protocol
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 71)

Abstract

Although Haemophilus influenzae genes may be transferred by plasmidmediated conjugation, this process is not discussed further below, as it has received little attention and is rarely used for strain construction. Conjugative plasmids are common in H. influenzae, as in other bacteria (1-3). The F plasmid of Escherichia coli can conjugate into H. influenzae cells (4) (and into many other cell types), and can then transfer by conjugation from one H. influenzae strain to another. As in E. coli, efficient transfer of chromosomal genes requires a region of homology between the plasmid and the chromosome. A method for F-mediated conjugation into H. influenzae is described by Deich and Green (4).

Keywords

Competent Cell Transformation Frequency Influenzae Strain Natural Competence Bald Patch 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Grant Poje
    • 1
  • Rosemary J. Redfield
    • 1
  1. 1.Department of ZoologyUniversity of British ColumbiaVancouverCanada

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