Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC
Over the last few decades, the use of swine as an animal model for human diseases in biomedical research has been steadily increasing because of similarities between the two species. The gnotobiotechnique, on the other hand, has been developed further since the beginning of the 20th century (1, 2, 3), stimulated by the need for an experimental model to study bacteria-host interactions in sterile laboratory animals, during the course of an infection with a defined pathogen. The combination of both aspects led to the development of a complex isolator system that made the delivery of piglets by cesarean section and their rearing in a self-contained unit possible, shielded from undesirable contaminating germs (4,5). In such a microbiologically well-defined environment, pathogen-host interactions can be studied without the influence of accompanying bacterial flora.
- 1.Küster E. (1912) Die keimfreie Züchtung von Säugetieren und ihre Bedeutung für die Erforschung der Körperfunktionen. Zbl. Bakteriol. 54, 55.Google Scholar
- 2.Reyniers J. A. (1941) Apparatus for a method of maintaining and working with biological specimens in a germfree controlled environment. US Patent 2244082.Google Scholar
- 12.Donohue-Rolfe A., Kondova I., Oswald S., Hutto D., and Tzipori S. (2000) Escherichia coli O157:H7 strains that express Shiga toxin (Stx) 2 alone are more neurotropic for gnotobiotic piglets than are isotypes producing only Stx1 or both Stx1 and Stx2. J. Infect. Dis. 181, 1825–1829.CrossRefPubMedGoogle Scholar
- 13.Waldmann K. H. (1988) Gnotobiotische Gewinnung und Haltung von Ferkeln der Rasse Göttinger Miniaturschwein. Tierärztl. Prax. 3(Suppl.), 84–92.Google Scholar
- 14.Ley F. J. (1976) Radiation sterilization of diets. J. Inst. Anim. Tech. 26, 87.Google Scholar