RT-PCR Protocols pp 83-92

Part of the Methods in Molecular Biology book series (MIMB, volume 193)

Quantitative RT-PCR

A Review of Current Methodologies
  • Caroline Joyce

Abstract

The powerful amplification potential of PCR has assured its use in the detection of low-abundance mRNA in cells and tissues. RT-PCR is currently the most sensitive technique available for mRNA detection and quantification. It can accurately quantify genes present at only a few hundred copies per sample, and has become the method of choice for the examination of gene expression. The technique consists of two parts: synthesis of cDNA from RNA by reverse transcription, and amplification of a specific cDNA by polymerase chain reaction (PCR). The method requires very little RNA and differs from Northern blotting because it is somewhat tolerant of degraded RNA, as long as the RNA is intact within the region of interest.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Caroline Joyce
    • 1
  1. 1.Department of BiochemistryUniversity HospitalCorkIreland

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