The Use of Permeabilized Cell Systems to Study Nuclear Transport

  • Amy M. Brownawell
  • James M. Holaska
  • Ian G. Macara
  • Bryce M. Paschal
Part of the Methods in Molecular Biology™ book series (MIMB, volume 189)


Nucleocytoplasmic transport occurs through nuclear pore complexes (NPC), macromolecular structures which span the nuclear envelope (1). NPCs contain aqueous channels with a diameter of ~9 nm, allowing ions, metabolites, and small proteins to passively diffuse between the nucleus and the cytoplasm (2). Most proteins and RNAs, however, exceed the ~60-kDa diffusion limit and are transported through the NPC by mechanisms that are saturable (3), energy-dependent (4), and signal-mediated (6).


Nuclear Import Nuclear Pore Complex Protein Kinase Inhibitor Nuclear Localization Sequence Potassium Acetate 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Amy M. Brownawell
    • 1
  • James M. Holaska
    • 2
  • Ian G. Macara
    • 1
  • Bryce M. Paschal
    • 3
  1. 1.Department of Pharmacology, Center for Cell SignalingUniversity of VirginiaCharlottesville
  2. 2.Department of MicrobiologyUniversity of VirginiaCharlottesville
  3. 3.Department of Biochemistry and Molecular Genetics, Center for Cell SignalingUniversity of VirginiaCharlottesville

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