Malaria Methods and Protocols pp 103-116

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 72)

Genotyping of Plasmodium spp.

Nested PCR
  • Georges Snounou

Abstract

It is now established that Plasmodium falciparum parasites found in any one area are highly diverse and that individual hosts, vertebrate and insect, are often concurrently infected by multiple parasite lines (for example, seerefs.1-4). Different parasite lines of the same Plasmodium species are also known to vary substantially with respect to parameters such as the parasitological and clinical course of the infection, the pattern of recrudescence, immunological cross-reactivity, susceptibility to drugs, and transmissibility by various vector species. The relationship between the complexity of the parasite populations and such pathological, immunological, and epidemiological factors is poorly understood and clearly merits further investigation (for example, seerefs.4-16).

References

  1. 1.
    Mercereau-Puijalon, O., Fandeur, T., Bonnefoy, S., Jacquemot, C., and Sarthou, J.-L. (1991) A study of the genomic diversity of Plasmodium falciparum in Senegal. 2. Typing by the use of the polymerase chain reaction. Acta. Trop. 49, 293–304.CrossRefPubMedGoogle Scholar
  2. 2.
    Felger, I., Tavul, L., Kabintik, S., Marshall, V. M., Genton, B., Alpers, M. P., and Beck, H.-P. (1994) Plasmodium falciparum: Extensive polymorphism in merozoite surface antigen 2 alleles in an area with endemic malaria in Papua New Guinea. Exp. Parasitol. 79, 106–116.CrossRefPubMedGoogle Scholar
  3. 3.
    Viriyakosol, S., Siripoon, N., Zhu, X. P., Jarra, W., Seugorn, A., Brown, K. N., and Snounou, G. (1994) Plasmodium falciparum: Selective growth of subpopulations from field samples following in vitro culture, as detected by the polymerase chain reaction}. Exp. Parasitol. 79, 517–525.CrossRefPubMedGoogle Scholar
  4. 4.
    Contamin, H., Fandeur, T., Rogier, C., Bonnefoy, S., Konate, L., Trape, J.-F., and Mercereau-Puijalon, O. (1996) Different genetic characteristics of Plasmodium falciparum isolates collected during successive clinical malaria episodes in Senegalese children. Am. J. Trop. Med. Hyg. 54, 632–643.PubMedGoogle Scholar
  5. 5.
    Babiker, H. A., Ranford-Cartwright, L. C., Currie, D., Charlwood, J. D., Billingsley, P., Teuscher, T., and Walliker, D. (1994) Random mating in a natural population of the malaria parasite Plasmodium falciparum. Parasitol. 109, 413–421.CrossRefGoogle Scholar
  6. 6.
    Paul, R. E. L., Packer, M. J., Walmsley, M., Lagog, M., Ranford-Cartwright, L. C., Paru, R., and Day, K. P. (1995) Mating patterns in malaria parasite populations of Papua New Guinea. Science 269, 1709–1711.CrossRefPubMedGoogle Scholar
  7. 7.
    Engelbrecht, F., Felger, I., Genton, B., Alpers, M., and Beck, H.-P. (1995) Plasmodium falciparum: malaria morbidity is associated with specific merozoite surface antigen 2 genotypes. Exp. Parasitol. 81, 90–96.CrossRefPubMedGoogle Scholar
  8. 8.
    Ntoumi, F., Contamin, H., Rogier, C., Bonnefoy, S., Trape, J.-F., and Mercereau-Puijalon, O. (1995) Age-dependent carriage of multiple Plasmodium falciparum merozoite surface antigen-2 alleles in asymptomatic malaria infections. Am. J. Trop. Med. Hyg. 52, 81–88.PubMedGoogle Scholar
  9. 9.
    Arez, A. P., Palsson, K., Pinto, J., Franco, A. S., Dinis, J., Jaenson, T. G. T., Snounou, G., and Do Rosario, V. E. (1997) Transmission of mixed malaria species and strains by mosquitoes, as detected by PCR, in a study area in Guinea-Bissau. Parassitol. 39, 65–70.Google Scholar
  10. 10.
    Färnert, A., Snounou, G., Rooth, I., and Björkman, A. (1997) Daily dynamics of Plasmodium falciparum subpopulations in asymptomatic children in a holoendemic area. Am. J. Trop. Med. Hyg. 56, 538–547.PubMedGoogle Scholar
  11. 11.
    Beck, H.-P., Felger, I., Huber, W., Steiger, S., Smith, T. A., Weiss, N. A., Alonso, P. L., and Tanner, M. (1997) Analysis of multiple Plasmodium falciparum infections in Tanzanian children during the phase III trial of the malaria vaccine SPf66. J. Inf. Dis. 175, 921–926.CrossRefGoogle Scholar
  12. 12.
    Snounou, G. and Beck, H.-P. (1998) The use of PCR-genotyping in the assessment of recrudescence or reinfection after antimalarial treatment Parasitol. Today 14, 462–467.CrossRefPubMedGoogle Scholar
  13. 13.
    Haywood, M., Conway, D. J., Weiss, H., Metzger, W., Alessandro, U. d., Snounou, G., Targett, G. A. T., and Greenwood, B. M. (1999) Reduction in the mean number of Plasmodium falciparum genotypes in Gambian children immunised with the malaria vaccine Spf66. Tran. R. Soc. Trop. Med. Hyg. 93, S1/65–S1/68.CrossRefGoogle Scholar
  14. 14.
    Arez, A. P., Snounou, G., Pinto, J., Sousa, C. A., Modiano, D., Ribeiro, H., et al. (1999) A clonal Plasmodium falciparum population in an isolated outbreak of malaria in the Republic of C abo Verde. Parasitol. 118, 347–355.CrossRefGoogle Scholar
  15. 15.
    Smith, T., Felger, I., Tanner, M., and Beck, H. P. (1999) Premunition in Plasmodium falciparum infection: insights from the epidemiology of multiple infections. Trans. R. Soc. Trop. Med. Hyg. 93 Suppl 1, 59–64.CrossRefPubMedGoogle Scholar
  16. 16.
    Snounou, G., Zhu, X., Viriyakosol, S., Jarra, W., Siripoon, N., Thaithong, S., and Brown, K. N. (1999) Biased distribution ofmsp1 and msp2 allelic variants in Plasmodium falciparum populations in Thailand. Trans. R. Soc. Trop. Med. Hyg. 93, 369–374.CrossRefPubMedGoogle Scholar
  17. 17.
    Ranford-Cartwright, L. C., Balfe, P., Carter, R., and Walliker, D. (1991) Genetic hybrids of Plasmodium falciparum identified by amplification of genomic DNA from single oocysts. Mol. Biochem. Parasitol. 49, 239–243.CrossRefPubMedGoogle Scholar
  18. 18.
    Snewin, V. A., Herrera, M., Sanchez, G., Scherf, A., Langsley, G., and Herrera, S. (1991) Polymorphism of the alleles of the merozoite surface antigens MSA1 and MS A2 in Plasmodium falciparum wild isolates from Colombia. Mol. Biochem. Parasitol. 49, 265–275.CrossRefPubMedGoogle Scholar
  19. 19.
    Wooden, J., Gould, E. E., Paull, A. T., and Sibley, C. H. (1992) Plasmodium falciparum: a simple polymerase chain reaction method for differentiating strains. Exp. Parasitol. 75, 207–212.CrossRefPubMedGoogle Scholar
  20. 20.
    Felger, I., Tavul, L., and Beck, H.-P. (1993) Plasmodium falciparum: A rapid technique for genotyping the merozoite surface protein 2. Exp. Parasitol. 77, 372–375.CrossRefPubMedGoogle Scholar
  21. 21.
    Contamin, H., Fandeur, T., Bonnefoy, S., Skouri, F., Ntoumi, F., and Mercereau-Puijalon, O. (1995) PCR typing of field isolates of Plasmodium falciparum. J. Clin. Microbiol. 33, 944–951.PubMedGoogle Scholar
  22. 22.
    Viriyakosol, S., Siripoon, N., Petcharapirat, C., Petcharapirat, P., Jarra, W., Thaithong, S., Brown, K. N., and Snounou, G. (1995) Genotyping of Plasmodium falciparum isolates by the polymerase chain reaction and potential uses in epidemiological studies. Bull. WHO 73, 85–95.PubMedGoogle Scholar
  23. 23.
    Cox-Singh, J., Mahayet, S., Abdullah, M. S., and Singh, B. (1997) Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction. Int. J. Parasitol. 27, 1575–1577.CrossRefPubMedGoogle Scholar
  24. 24.
    Färnert, A., Arez, A. P., Correia, A. T., Björkman, A., Snounou, G., and Do Rosário, V. (1999) Sampling and storage of blood and the detection of malaria parasites by polymerase chain reaction. Trans. R. Soc. Trop. Med. Hyg. 93, 50–53.CrossRefPubMedGoogle Scholar
  25. 25.
    Schriefer, M. E., Sacci, J. B., Jr., Wirtz, R. A., and Azad, A. F. (1991) Detection of polymerase chain reaction-amplified malarial DNA in infected blood and individual mosquitoes. Exp. Parasitol. 73, 311–316.CrossRefPubMedGoogle Scholar
  26. 26.
    Siridewa, K., Karunanayake, E. H., and Chandrasekharan, N. V. (1996) Polymerase chain reactionbased technique for the detection of Wuchereria bancrofti in human blood samples, hydrocele fluid, and mosquito vectors. Am. J. Trop. Med. Hyg. 54, 72–76.PubMedGoogle Scholar
  27. 27.
    Arez, A. P., Lopes, D., Pinto, J., Franco, A. S., Snounou, G., and Do Rosário, V. E. (2000) Plasmodium sp.: optimal protocols for PCR amplification of low parasite numbers from mosquito (Anopheles sp.) samples. Exp. Parasitol., 94, 269–272.CrossRefPubMedGoogle Scholar
  28. 28.
    Borre, M. B., Dziegiel, M., Høgh, B., Petersen, E., Rieneck, K., Riley, E., et al. (1991) Primary structure and localization of a conserved immunogenic Plasmodiumfalciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle. Mol. Biochem. Parasitol. 49, 119–131.CrossRefPubMedGoogle Scholar
  29. 29.
    Miller, L. H., Roberts, T., Shahabuddin, M., and McCutchan, T. F. (1993) Analysis of sequence diversity in the Plasmodium falciparum merozoite surface protein-1 (MSP-1). Mol. Biochem. Parasitol. 59, 1–14.CrossRefPubMedGoogle Scholar
  30. 30.
    Smythe, J. A., Peterson, M. G., Coppel, R. L., Saul, A. J., Kemp, D. J., and Anders, R. F. (1990) Structural diversity in the 45-kilodalton merozoite surface antigen of Plasmodiumfalciparum. Mol. Biochem. Parasitol. 39, 227–234.CrossRefPubMedGoogle Scholar
  31. 31.
    Snounou, G., Viriyakosol, S., Zhu, X. P., Jarra, W., Pinheiro, L., Do Rosario, V. E., et al. (1993) High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol. Biochem. Parasitol. 61, 315–320.CrossRefPubMedGoogle Scholar
  32. 32.
    Snounou, G., Pinheiro, L., Gonçalves, A., Fonseca, L., Dias, F., Brown, K. N., et al. (1993) The importance of sensitive detection of malaria parasites in the human and insect hosts in epidemiological studies, as shown by the analysis of field samples from Guinea Bissau. Trans. R. Soc. Trop. Med. Hyg. 87, 649–653.CrossRefPubMedGoogle Scholar
  33. 33.
    Hill, W. G. and Babiker, H. A. (1995) Estimation of numbers of malaria clones in blood samples. Proc. R. Soc. Lond. B 262, 249–257.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Georges Snounou
    • 1
  1. 1.Bio-medical ParasitologyInstitut PasteurParisFrance

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