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Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function

  • Nigel Holder
  • Qiling Xu
Part of the Methods in Molecular Biology™ book series (MIMB, volume 97)

Abstract

The use of microinjection to study gene function in the zebrafish has become widespread in recent years. This includes ectopic expression of genes by introducing DNA (1) or RNA into embryos or injection of blocking molecules, such as RNA encoding truncated proteins (2) or antibodies, to perturb the function of endogenouse gene products (3,4). The method involves microinjection of DNA and RNA molecules into the cytoplasm of one-cell-stage embryos using pressure microinjector and micromanipulator as described below.

Keywords

Study Gene Function Embryo Medium Injection Chamber Suitable Restriction Enzyme Blunt Forceps 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Nigel Holder
    • 1
  • Qiling Xu
    • 2
  1. 1.Department of Anatomy and Developmental BiologyUniversity College LondonUK
  2. 2.National Institute for Medical ResearchLondonUK

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