Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function
The use of microinjection to study gene function in the zebrafish has become widespread in recent years. This includes ectopic expression of genes by introducing DNA (1) or RNA into embryos or injection of blocking molecules, such as RNA encoding truncated proteins (2) or antibodies, to perturb the function of endogenouse gene products (3,4). The method involves microinjection of DNA and RNA molecules into the cytoplasm of one-cell-stage embryos using pressure microinjector and micromanipulator as described below.
KeywordsStudy Gene Function Embryo Medium Injection Chamber Suitable Restriction Enzyme Blunt Forceps
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