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Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme

  • Chang Kee Lim
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Chromatographic techniques are widely used for the analysis of heme and its precursors. Recent and continuing improvements in column packing materials for high-performance liquid chromatography (HPLC) have led to much better column efficiency and resolution. There have also been great advances in the direct coupling of liquid chromatography (LC), including capillary electrophoresis (CE), to mass spectrometry (MS) to provide highly sensitive and specific methods of analysis.

Keywords

Elution Order Ammonium Acetate Buffer Porphyria Cutanea Tarda Hydrophobic Surface Area Sodium Amalgam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bonnett, R., AA. Charalambides, K. Jones, I A. Magnus, and R.J. Ridge. 1978. The direct determination of porphyrin carboxylic acids. High-pressure liquid chromatography with solvent systems containing phase-transfer agents. Biochem. J. 173:693–695.PubMedGoogle Scholar
  2. 2.
    Chiang, S.C.C. and S.F.Y. Li. 1997. Separation of porphyrins by capillary electrophoresis in fused-silica and ethylene vinyl acetate copolymer capillaries with visible absorbance detection. Biomed. Chromatogr. 11:366–370.PubMedCrossRefGoogle Scholar
  3. 3.
    Li, F., C.K. Lim, and T.J. Peters. 1987. Separation and characterization of pentacarboxylic porphyrinogen isomers by high-performance liquid chromatography with electrochemical detection. Biochem. J. 243:421–423.Google Scholar
  4. 4.
    Li, F., C.K. Lim, and T.J. Peters. 1989. Preparation, high-performance liquid chromatographic separation and characterization of hexacarboxylic porphyrinogens. J. Chromatogr. 461:353–359.PubMedCrossRefGoogle Scholar
  5. 5.
    Lim, C.K., F. Li, and T.J. Peters. 1986. High-performance liquid chromatography of uroporphyrinogen and coproporphyrinogen isomers with amperometric detection. Biochem. J. 234:629–633.PubMedGoogle Scholar
  6. 6.
    Lim, C.K., F. Li, and T.J. Peters. 1987. High-performance liquid chromatography of type-III heptacarboxylic porphyrinogen isomers. Biochem. J. 247:229–232.PubMedGoogle Scholar
  7. 7.
    Lim, C.K., F. Li, and T.J. Peters. 1988. High-performance liquid chromatography of porphyrins. A review. J. Chromatogr. Biomed. Appl. 429:123–153.CrossRefGoogle Scholar
  8. 8.
    Lim, C.K. and T.J. Peters. 1984. Urine and faecal porphyrin profiles by reversed-phase high-performance liquid chromatography in the porphyrias. Clin. Chim. Acta 139:55–63.PubMedCrossRefGoogle Scholar
  9. 9.
    Lim, C.K., M.A. Razzaque, J. Luo, and P.B. Farmer. 2000. Isolation and characterization of protoporphyrin glycoconjugates from rat Harderian gland by HPLC, capillary electrophoresis and HPLC/electrospray ionization MS. Biochem. J. 347:757–761.PubMedCrossRefGoogle Scholar
  10. 10.
    Lim, C.K., J.M. Rideout, and T.J. Peters. 1984. High-performance liquid chromatography of dicarboxylic porphyrins and metalloporphyrins: retention behaviour and biomedical applications. J. Chromatogr. 377:333–341.CrossRefGoogle Scholar
  11. 11.
    Lim, C.K., J.M. Rideout, and D.M. Samson. 1979. Determination of 5-aminolaevulinic acid and porphobilinogen by high-performance liquid chromatography. J. Chromatogr. 185:605–611.PubMedCrossRefGoogle Scholar
  12. 12.
    Lord, G.A., J.L. Luo, and C.K. Lim. 1999. Capillary zone electrophoresis/mass spectrometry of 5-aminolaevulinic acid and porphobilinogen. Rapid. Comm. Mass Spec. 74:314–316.Google Scholar
  13. 13.
    Luo, J.L., J. Deka, and C.K. Lim. 1996. Determination of 5-aminolaevulinic acid dehydratase activity in erythrocytes and porphobilinogen in urine by micellar electrokinetic capillary chromatography. J. Chromatogr. 722:353–357.CrossRefGoogle Scholar
  14. 14.
    Luo, J. and C.K. Lim. 1993. Order of uroporphyrinogen III decarboxylation on incubation of porphobilinogen and uroporphyrinogen III with erythrocyte uroporphyrinogen decarboxylase. Biochem. J. 289:529–532.PubMedGoogle Scholar
  15. 15.
    Luo, J. and C.K. Lim. 1995. Isolation and characterization of new porphyrin metabolites in human porphyria cutanea tarda and in rats treated with hexachlorobenzene by HPTLC, HPLC and liquid secondary ion mass spectrometry. Biomed. Chromatogr. 9:113–122.PubMedCrossRefGoogle Scholar
  16. 16.
    Mauzerall, D. and S. Granick. 1956. The occurrence and determination of δ-aminolevulinic acid and porphobilinogen in urine. J. Biol. Chem. 219:435–446.PubMedGoogle Scholar
  17. 17.
    Meyer, H.D., W. Vogt, and K. Jacob. 1984. Improved separation and detection of free porphyrins by high-performance liquid chromatography. J. Chromatogr. 290:207–213.PubMedCrossRefGoogle Scholar
  18. 18.
    Rideout, J.M., D.J. Wright, and C.K. Lim. 1983. High performance liquid chromatography of uroporphyrin isomers. J. Liq. Chromatogr. 6:383–394.CrossRefGoogle Scholar
  19. 19.
    Rossi, E. and D.H. Curnow. 1986. Porphyrins, Ch. 10, p. 261–303. In C.K. Lim (Ed.), HPLC of Small Molecules. IRL Press, Oxford.Google Scholar
  20. 20.
    Weinberger, R., E. Sapp, and S. Moring. 1990. Capillary electrophoresis of urinary porphyrins with absorbance and fluorescence detection. J. Chromatogr. 516:217–285.CrossRefGoogle Scholar

Copyright information

© Humana Press, Totowa, NJ 2002

Authors and Affiliations

  • Chang Kee Lim
    • 1
  1. 1.MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck CollegeUniversity of LondonLondonEngland, UK

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