Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme

  • Chang Kee Lim
Part of the Springer Protocols Handbooks book series (SPH)


Chromatographic techniques are widely used for the analysis of heme and its precursors. Recent and continuing improvements in column packing materials for high-performance liquid chromatography (HPLC) have led to much better column efficiency and resolution. There have also been great advances in the direct coupling of liquid chromatography (LC), including capillary electrophoresis (CE), to mass spectrometry (MS) to provide highly sensitive and specific methods of analysis.


Elution Order Ammonium Acetate Buffer Porphyria Cutanea Tarda Hydrophobic Surface Area Sodium Amalgam 
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Copyright information

© Humana Press, Totowa, NJ 2002

Authors and Affiliations

  • Chang Kee Lim
    • 1
  1. 1.MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck CollegeUniversity of LondonLondonEngland, UK

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