Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice

  • Ahmed Mansouri
Part of the Methods in Molecular Biology™ book series (MIMB, volume 175)


A decade ago the first alteration of a gene using embryonic stem (ES) cells was performed in the mouse (1). Since then ES cells have become a powerful tool to generate mutant mice for the analysis of gene function. These mutant mice (also called knockout mice) have provided new insights into normal and pathologic development to improve our knowledge and open new avenues into diagnostic and therapeutic fields.


Embryonic Stem Embryonic Stem Cell Leukemia Inhibitory Factor Tissue Culture Dish Embryonic Stem Cell Line 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ahmed Mansouri
    • 1
  1. 1.Department of Molecular and Cell BiologyMax-Planck-Institute for Biophysical ChemistryGöttingenGermany

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