Elimination of Cell Types from Mixed Neural Cell Cultures

  • Richard M. Devon
Part of the Springer Protocols Handbooks book series (SPH)


The isolation and purification, or enrichment, of neuronal or glial cell types from central nervous system (CNS) tissues require the application of one of several strategies. It is possible to separate a particular cell type, based on its cell mass or presence of specific cell surface antigens. Differential gradients and centrifugal force (Pretlow and Pretlow 1983, see also  Chapter 10), binding a variety of ligands, including magnets, to specific cell surface antigens can be utilized to isolate a cell type before the cells have been planted in cell cultures. Another approach is to plant a mixed cell population in culture, then proceed with purification of the culture, either by elimination of a certain cell type, or by encouraging others to proliferate and overgrow a culture. Selection of a cell type in culture requires the addition of compounds or substitution of specific nutrients to the growth medium.


Cerebellar Granule Neuron Nonneuronal Cell Antimitotic Agent Astroglia Culture Adenine Arabinoside 
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Further Reading

  1. Alosi, F., Agresti, C, D’Urso, D., and Levi, G. (1988), Differentiation of bipotential glial precursors into oligodendrocytes is promoted by the interaction with type-1 astrocytes in cerebellar cultures. Proc. Nat. Acad. Sci. USA 85, 6167–6171.CrossRefGoogle Scholar
  2. Banker, G. and Goslin, K. (1992), Primary dissociated cell cultures of neural tissues, in: Culturing Nerve Cells, Banker, G. and Goslin, K., eds., MIT Press, Cambridge, MA, pp. 41–74.Google Scholar
  3. Bottenstein, J. E., Hunter, S. F., and Seidel, M. (1988), CNS neuronal cell-line-derived factors regulate gliogenesis in neonatal rat brain cultures. J. Neurosci. Res. 20, 291–303.PubMedCrossRefGoogle Scholar
  4. Cholewinski, A. J., Reid, J. C, McDermott, A. M., and Wilkin, G. P. (1989), Purification of astroglial-cell cultures from rat spinal cord: the use of D-valine to inhibit fibroblast growth. Neurochem. Int. 15, 365–369.PubMedCrossRefGoogle Scholar
  5. Espinosa de los Monteros, A., Roussel, G., Neskovic, N. M., and Nussbaum, J. L. (1988), A chemically defined medium for the culture of mature oligodendrocytes. J. Neurosci. Res. 19, 202–211.PubMedCrossRefGoogle Scholar
  6. Estin, C. and Vernadakis, A. (1989), Purification of glial cell cultures: Medium chemical conditions for the culture of astrocytes, in: A Dissection and Tissue Culture Manual of the Nervous System, Shahar, A., de Vellis, J., Vernadakis, A., and Haber, B., eds., Alan R. Liss, New York, pp. 112–114.Google Scholar
  7. Fields, K. (1985), Neuronal and glial surface antigens on cells in culture, in: Cell Culture in the Neurosciences, Bottenstein, J. E. and Sato, G., eds., Plenum, New York, pp. 45–93.Google Scholar
  8. Hao, C., Richardson, A., and Fedoroff, S. (1990), Production of colony-stimulating factor-1 (CSF-1) by mouse astroglia in vitro. J. Neurosci. Res. 27, 314–323.PubMedCrossRefGoogle Scholar
  9. Hardy, R. and Reynolds, R. (1991), Proliferation and differentiation potential of rat forebrain oligodendroglial progenitors both in vitro and in vivo. Development 111, 1061–1080.PubMedGoogle Scholar
  10. Honneger, P. and Werfflei, P. (1988), Use of aggregating cell cultures for toxicological studies. Experientia 44, 817–822.CrossRefGoogle Scholar
  11. Martin, D. P., Wallace, T. L., and Johnson, E. M. (1990), Cytosine arabinoside kills postmitotic neurons in a fashion resembling trophic factor deprivation: evidence that a deoxycytidine-dependent process may be required for nerve growth factor signal transduction. J. Neurosci. 10, 184–193.PubMedGoogle Scholar
  12. Neuhaus, J. and Fedoroff, S. (1994), Development of microglia in mouse neopallial cell cultures. Glia 11, 11–17.PubMedCrossRefGoogle Scholar
  13. Owen, C. S. (1983), Magnetic Cell Sorting, in Cell Separation: Methods and Selected Applications, Pretlow, T. G. II and Pretlow, T. P., eds., vol. 2 Academic, New York, pp. 127–143.Google Scholar
  14. Pretlow, T. G. and Pretlow, T. P., eds. (1983), Cell Separation: Methods and Selected Applications, vol. 2. Academic, London.Google Scholar
  15. Seil, F. J., Drake-Baumann, R., Herndon, R. M., and Leiman, A. L. (1992), Cytosine arabinoside effects in mouse cerebellar cultures in the presence of astrocytes. Neuroscience 51, 149–158.PubMedCrossRefGoogle Scholar
  16. Shine, H. D. (1989), Efficient photo-induced killing of dividing cells in neural cultures with 5-bromodeoxyuridine and Hoechst dye 33258, in: A Dissection and Tissue Culture Manual of the Nervous System, Shahar, A., de Vellis, J., Vernadakis, A., and Haber, B., eds., Alan R. Liss, New York, pp. 169–171.Google Scholar
  17. Theile, D. L. and Lipsky, P. E. (1985), Modulation of human natural killer cell function by L-leucine methyl ester: monocyte-dependent depletion from human peripheral blood mononuclear cells. J. Immunol. 134, 786–793.Google Scholar
  18. Wallace, T. L. and Johnson, E. M. (1989), Cytosine arabinoside kills postmitotic neurons: Evidence that deoxycytidine may have a role in neuronal survival that is independent of DNA synthesis. J. Neurosci. 9, 115–124.PubMedGoogle Scholar
  19. Wiesinger, H., Schuricht, B., and Hamprecht, B. (1991), Replacement of glucose by sorbitol in growth medium causes selection of astroglial cells from heterogeneous primary cultures derived from newborn mouse brain. Brain Res. 550, 69–76.PubMedCrossRefGoogle Scholar
  20. Woodroofe, M. N. and Cuzner, M. L. (1995), Microglia: the tissue macrophage of the CNS, in: Neural Cell Culture: A Practical Approach, Cohen, J. and Wilkin, G. P., eds., Oxford University Press, Oxford, UK, pp.107–119.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Richard M. Devon
    • 1
  1. 1.Department of Oral Biology, College of DentistryUniversity of SaskatchewanSaskatoonCanada

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