- 2.8k Downloads
The premise of colony culturing is the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony or clone. Colony cultures can be initiated either from a disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures or cell lines. Colonies, especially ones initiated directly from tissues, are not all identical. They vary in the morphology of their cells, and in their size and compactness. The morphology and size of the colony depends on the kinds of cells plated, interactions between cells, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions to which the cells are subjected.
KeywordsCell Coloni Culture Vessel Plate Efficiency Limit Dilution Method Mouse Neopallium
- Clarke, J., Thorpe, R., and Davis, J. (1994), Cloning, in: Basic Cell Culture. A Practical Approach., Davis, J. M., ed., Oxford University Press, Oxford, UK.Google Scholar
- Fedoroff, S. (1984), A method for the study of neural cell lineages based on colony culture and transplantation of cultured cells into the CNS, in: Developmental Neuroscience: Physiological, Pharmacological and Clinical Aspects, Caciagle, F., Giacobini, E., Paoletti, R., eds., Elsevier, Amsterdam, pp. 373–376.Google Scholar
- Lefkovits, I. and Waldmann, H. (1979), Limiting Dilution Analysis of Cells in the Immune System. Cambridge University Press, Cambridge, UK.Google Scholar