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Olfactory Ensheathing Cell Cultures

  • Ronald Doucette
Protocol
  • 2.8k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Over the past several years, neuroscientists have developed a considerable interest in a glial cell found only in the first cranial nerve. These glial cells, which are referred to as “olfactory ensheath-ing cells,” provide ensheathment for the unmyelinated axons of the olfactory nerve (Doucette, 1984, Doucette, 1986, 11,1; Raisman, 1985). Two major reasons why these cells have become so popular are their ability, first, to promote the long-distance growth of regenerating axons in the adult mammalian central nervous system (CNS) (Li et al., 1997; Perez-Bouza et al., 1998; Ramon-Cueto and Nieto-Sampedro, 1994; Ramon-Cueto et al., 1998; Smale et al., 1996), and second, to remyelinate spinal cord axons in adult rats (Franklin et al., 1996; Imaizumi et al., 1998). For an overview of the cell biology of these glial cells, the reader is referred to several reviews on the subject (Doucette, 1990, Doucette, 1995; Franklin and Barnett, 1997; Ramon-Cueto and Avila, 1998; Ramon-Cueto and Valverde, 1995). The present chapter chiefly addresses those cell biological aspects that are pertinent to obtaining purified cell cultures of ensheathing cells from the fetal rat olfactory bulb.

Keywords

Abdominal Wall Olfactory Bulb Uterine Horn Olfactory Nerve Unmyelinated Axon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Further Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ronald Doucette
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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