Olfactory Ensheathing Cell Cultures

  • Ronald Doucette
Part of the Springer Protocols Handbooks book series (SPH)


Over the past several years, neuroscientists have developed a considerable interest in a glial cell found only in the first cranial nerve. These glial cells, which are referred to as “olfactory ensheath-ing cells,” provide ensheathment for the unmyelinated axons of the olfactory nerve (Doucette, 1984, Doucette, 1986, 11,1; Raisman, 1985). Two major reasons why these cells have become so popular are their ability, first, to promote the long-distance growth of regenerating axons in the adult mammalian central nervous system (CNS) (Li et al., 1997; Perez-Bouza et al., 1998; Ramon-Cueto and Nieto-Sampedro, 1994; Ramon-Cueto et al., 1998; Smale et al., 1996), and second, to remyelinate spinal cord axons in adult rats (Franklin et al., 1996; Imaizumi et al., 1998). For an overview of the cell biology of these glial cells, the reader is referred to several reviews on the subject (Doucette, 1990, Doucette, 1995; Franklin and Barnett, 1997; Ramon-Cueto and Avila, 1998; Ramon-Cueto and Valverde, 1995). The present chapter chiefly addresses those cell biological aspects that are pertinent to obtaining purified cell cultures of ensheathing cells from the fetal rat olfactory bulb.


Abdominal Wall Olfactory Bulb Uterine Horn Olfactory Nerve Unmyelinated Axon 
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Further Reading

  1. Barnett, S.C., Hutchins, A.-M., and Noble, M. (1993), Purification of olfactory nerve ensheathing cells from the olfactory bulb. Dev. Biol 155, 337–350.PubMedCrossRefGoogle Scholar
  2. Bottenstein, J. (1985), Growth and differentiation of neural cells in defined media, in: Cell Culture in the Neurosciences, Bottenstein, J. E. and Sato, G., eds., Plenum, New York, pp. 3–43.Google Scholar
  3. Butler, H. and Juurlink, B. (1987), An Atlas for Staging Mammalian and Chick Embryos. CRC, Boca Raton, FL.Google Scholar
  4. Chuah, M. I. and Au, C. (1993), Cultures of ensheathing cells from neonatal rat olfactory bulbs. Brain Res., 601, 213–220.PubMedCrossRefGoogle Scholar
  5. Chuah, M. I. and Au, C. (1994), Olfactory cell cultures on ensheathing cell monolayers. Chem. Senses 19, 25–34.PubMedCrossRefGoogle Scholar
  6. Devon, R. and Doucette, R. (1992), Olfactory ensheathing cells myelinate dorsal root ganglion neurites. Brain Res. 589, 175–179.PubMedCrossRefGoogle Scholar
  7. Devon, R. and Doucette, R. (1995), Olfactory ensheathing cells do not require L-ascorbic acid in vitro to assemble a basal lamina or to myelinate dorsal root ganglion neurites. Brain Res. 688, 223–229.PubMedCrossRefGoogle Scholar
  8. Doucette, R. (1984), The glial cells in the nerve fiber layer of the rat olfactory bulb. Anat. Rec. 210, 385–391.PubMedCrossRefGoogle Scholar
  9. Doucette, R. (1986), Astrocytes in the olfactory bulb, in: Astrocytes, vol. 1. Edited by Fedoroff, S. and Vernadakis, A., eds., Academic, New York, pp. 293–310.Google Scholar
  10. Doucette, R. (1990), Glial influences on axonal growth in the primary olfactory system. Glia 3, 433–449.PubMedCrossRefGoogle Scholar
  11. Doucette, R. (1993a), Glial cells in the nerve fiber layer of the main olfactory bulb of embryonic and adult mammals. Microscopy Res. and Technique 24, 113–130.CrossRefGoogle Scholar
  12. Doucette, R. (1993b), Glial progenitor cells of the nerve fiber layer of the olfactory bulb: effect of astrocyte growth media. J. Neurosci. Res. 35, 274–287.PubMedCrossRefGoogle Scholar
  13. Doucette, R. (1995), Olfactory ensheathing cells: Potential for glial cell transplantation into areas of CNS injury. Histol. Histopathology 10, 503–507.Google Scholar
  14. Doucette, R. and Devon, R. (1994), Media that support the growth and differentiation of oligodendrocytes do not induce olfactory ensheathing cells to express a myelinating phenotype. Glia 10, 296–310.PubMedCrossRefGoogle Scholar
  15. Doucette, R. and Devon, R. (1995), Elevated intracellular levels of cAMP induce olfactory ensheathing cells to express GAL-C and GFAP but not MBP. Glia 13, 130–140.PubMedCrossRefGoogle Scholar
  16. Franceschini, I. A. and Barnett, S. C. (1996), Low-affinity NGF-receptor and E-N-CAM expression define two types of olfactory nerve ensheathing cells that share a common lineage. Dev. Biol. 173, 327–343.PubMedCrossRefGoogle Scholar
  17. Franklin, R. J. M. and Barnett, S. C. (1997), Do olfactory glia have advantages over Schwann cells for CNS repair? J. Neurosci. Res. 50, 665–672.PubMedCrossRefGoogle Scholar
  18. Franklin, R. J. M., Gilson, J. M., Franceschini, I. A., and Barnett, S. C. (1996), Schwann cell-like myelination following transplantation of an olfactory bulb-ensheathing cell line into areas of demyelination in the adult CNS. Glia 17, 217–224.PubMedCrossRefGoogle Scholar
  19. Gudinocabrera, G. and Nieto-Sampedro, M. (1996), Ensheathing cells: large scale purification from adult olfactory bulb, freeze-preservation and migration of transplanted cells in adult brain. Restor. Neurol. Neurosci. 10, 25–34.Google Scholar
  20. Imaizumi, T., Lankford, K. L., Waxman, S. G., Greer, C. A., and Kocsis, J. D. (1998), Transplanted olfactory ensheathing cells remyelinate and enhance axonal conduction in the demyelinated dorsal columns of the rat spinal cord. J. Neurosci. 18, 6176–6185.PubMedGoogle Scholar
  21. Li, Y, Field, P. and Raisman, G. (1998), Regeneration of adult rat corticospinal axons induced by transplanted olfactory ensheathing cells. J. Neurosci. 18, 10,514–10,524.PubMedGoogle Scholar
  22. Li, Y, Field, P. M., and Raisman, G. (1997), Repair of adult rat corticospinal tract by transplants of olfactory ensheathing cells. Science 277, 2000–2002.PubMedCrossRefGoogle Scholar
  23. Perez-Bouza, A., Wigley, C. B., Nacimiento, W., Noth, J., and Brook, G. A. (1998), Spontaneous orientation of transplanted olfactory glia influences axonal regeneration [In Process Citation]. Neuroreport 9, 2971–2975.PubMedCrossRefGoogle Scholar
  24. Raisman, G. (1985), Specialized neuroglial arrangement may explain the capacity of vomeronasal axons to reinnervate central neurons. Neuroscience 14, 237–254.PubMedCrossRefGoogle Scholar
  25. Ramon-Cueto, A. and Avila, J. (1998), Olfactory ensheathing glia: properties and function. Brain Res. Bull. 46, 175–187.PubMedCrossRefGoogle Scholar
  26. Ramon-Cueto, A. and Nieto-Sampedro, M. (1992), Glial cells from adult rat olfactory bulb: Immunocytochemical properties of pure cultures of ensheathing cells. Neuroscience 47, 213–220.PubMedCrossRefGoogle Scholar
  27. Ramon-Cueto, A. and Nieto-Sampedro, M. (1994), Regeneration into the spinal cord of transected dorsal root axons is promoted by ensheathing glia transplants. Exp. Neurol. 127, 232–244.PubMedCrossRefGoogle Scholar
  28. Ramon-Cueto, A., Perez, J., and Nieto-Sampedro, M. (1993), In vitro enfolding of olfactory neurites by p75 NGF receptor positive ensheathing cells from adult rat olfactory bulb. Eur. J. Neurosci. 5, 1172–1180.PubMedCrossRefGoogle Scholar
  29. Ramon-Cueto, A., Plant, G. W., Avila, J., and Bunge, M. B. (1998), Long-distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants. J. Neurosci. 18, 3803–3815.PubMedGoogle Scholar
  30. Ramon-Cueto, A. and Valverde, F. (1995), Olfactory bulb ensheathing glia: a unique cell type with axonal growth-promoting properties. Glia 14, 163–173.PubMedCrossRefGoogle Scholar
  31. Smale, K. A., Doucette, R., and Kawaja, M. D. (1996), Implantation of olfactory ensheathing cells in the adult rat brain following fimbria-fornix transection. Exp. Neurol. 137, 225–233.PubMedCrossRefGoogle Scholar
  32. Sommer, I. and Schachner, M. (1981), Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: An immunological study in the central nervous system. Dev. Biol 83, 311–327.PubMedCrossRefGoogle Scholar
  33. Theiler, K. (1972), The House Mouse. Springer-Verlag, Berlin.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Ronald Doucette
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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