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Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections

  • Catherine Rabouille
Part of the Methods in Molecular Biology™ book series (MIMB, volume 117)

Abstract

A new challenge facing electron microscopists in the early 1980s was to combine their experience, taste, and knowledge of the cell ultrastructure with the newly developed antibodies (1) in order to expand morphology into biochemistry and “to bridge the gulf between the so called sciences of matter and science of form” (2). The preparation of samples for conventional electron microscopy is harsh. The infiltration of the samples with Epon or Araldite resins after the aldehyde fixation, the osmium postfixation, and the dehydration with ethanol and propylene oxide do not allow antigenicity to be preserved (except in some rare cases). New, softer methods for preserving some antigenicity along with a reasonable morphology were developed, allowing visualization of antigens (mainly proteins) on sections.

Keywords

Golgi Apparatus Gold Particle Relative Distribution Linear Density Label Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Catherine Rabouille
    • 1
  1. 1.Cell BiologyImperial Cancer Research FundLondon

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