Primer3 on the WWW for General Users and for Biologist Programmers

  • Steve Rozen
  • Helen Skaletsky
Part of the Methods in Molecular Biology™ book series (MIMB, volume 132)


Designing PCR and sequencing primers are essential activities for molecular biologists around the world. This chapter assumes acquaintance with the principles and practice of PCR, as outlined in, for example, refs. 1, 2, 3, 4.


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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Steve Rozen
    • 1
  • Helen Skaletsky
    • 1
  1. 1.Whitehead Institute for Biomedical ResearchCambridge

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