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Glycoprofiling Purified Glycoproteins Using Surface Plasmon Resonance

  • Angeliki Fotinopoulou
  • Graham A. Turner
Protocol
  • 71 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The carbohydrate part of glycoproteins can define several of their biological properties, including the clearance rate, immunogenicity, thermal stability, solubility, the specific activity, and conformation (1). Often, small differences in the composition of the sugar side chains of a glycoprotein can affect the biological properties (2). Characterization of oligosaccharide structures on glycoproteins is essential for glycoprotein therapeutic products, because inflammatory and systemic responses may arise if glycosylation of the product is different from that of the native substance.

Keywords

Surface Plasmon Resonance Sodium Acetate Lectin Binding Thin Gold Film Oligosaccharide Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Angeliki Fotinopoulou
    • 1
  • Graham A. Turner
    • 2
  1. 1.Department of Clinical Biochemistry, The Medical SchoolUniversity of NewcastleNewcastle upon TyneUK
  2. 2.Department of Clinical Biochemistry, The Medical SchoolUniversity of NewcastleNew Castle upon TyneUK

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