N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)
- 82 Downloads
The vast array of possible N-linked oligosaccharides demands high-resolution HPLC columns for their purification (1,2). Reverse-phase (C18) and normal-phase (NH2) columns have been used for the separation (singly or in concert) of many N-linked oligosaccharides. The porous graphitized carbon (PGC) column described in Chapter 114 for O-linked alditol separation will give improved N-linked oligosaccharide resolution over C18 columns, and has the advantage of using salt-free buffers for preparative work (3,4).
KeywordsVast Array Control Software Reductive Amination Preparative Work Pulse Amperometric Detection
- 2.Kakehi, K. and Honda, S. (1993) Analysis of carbohydrates in glycoproteins by high performance liquid chromatography and high performance capillary electrophoresis, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 81–98.CrossRefGoogle Scholar