Enzymatic Release of O-and N-Linked Oligosaccharide Chains
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Enzymes involved in both the synthesis and degradation of glycoconjugates are highly specific for monosaccharide, linkage position, and anomeric configuration factors further away in the oligosaccharide sequence or protein. Not withstanding this, endo- and exoglycosidases are extremely useful tools in structural analysis. The RAAM technique has automated the use of exoglycosidase digestion (Oxford Glycosystems, Abingdon, UK). Here we discuss the release of intact oligosaccharide chains from proteins that can be further analyzed for separate functions (1,2).
KeywordsSialic Acid Potassium Dihydrogen Sialic Acid Residue Separate Function Digestion Buffer