Chemical Release of O-Linked Oligosaccharide Chains

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Part of the Springer Protocols Handbooks book series (SPH)


O-linked oligosaccharides having the core sequences shown below can be released specifically from protein via a β-elimination reaction catalyzed by alkali. The reaction is usually carried out with concomitant reduction to prevent peeling, a reaction caused by further β-elimination around the ring of 3-substituted monosaccharides (1). The reduced oligosaccharides can be specifically bound by solid sorbent extraction on phenylboronic acid (PBA) columns (2).


Boronic Acid Core Sequence Concomitant Reduction Cation Exchange Resin Sugar Chain 
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  1. 1.
    Hounsell, E. F. (1994) Physicochemical analyses of oligosaccharides determinants of glycoproteins. Adv. Carbohyd. Chem. Biochem. 30, 311–350.CrossRefGoogle Scholar
  2. 2.
    Stoll, M. S. and Hounsell, E. F. (1988) Selective purification of reduced oligosaccharides using a phenylboronic acid bond elut column: potential application in HPLC, mass spectrometry, reductive amination procedures and antigenic/serum analysis. Biomed. Chromatogr. 2, 249–253.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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