Staining of Glycoproteins/Proteoglycans in SDS-Gels

  • Holger J. Møller
  • Jørgen H. Poulsen
Part of the Springer Protocols Handbooks book series (SPH)


Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of molecular weight determinations. In this chapter, staining methods are described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.


Alcian Blue Periodic Acid Molecular Weight Determination Silver Solution Automatic Staining 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Holger J. Møller
    • 1
  • Jørgen H. Poulsen
    • 1
  1. 1.Department of Clinical BiochemistryAarhus University HospitalAarhusDenmark

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